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Microscopy Masters asks one thing of citizen scientists: Find proteins in electron microscope images. The task will probably give participants new appreciation for biologists who decipher the structures of teeny,
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, tiny molecules. It’s not easy.
The goal of the?online project, created by researchers at the Scripps Research Institute in La Jolla, Calif.,
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, is to improve biologists’ ability to construct detailed,
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, three-dimensional models of proteins.
Using cryo-electron microscopy — which involves freezing,
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, then imaging a sample — the researchers have taken thousands of photos of their current target,
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, a protein complex involved in breaking down other, unwanted proteins. Each image contains 10 to 100 copies of the complex. It takes that many images to capture a protein from every angle. Once the 2-D images are stitched together, researchers can reconstruct the protein’s globular,
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, 3-D shape at near-atomic resolution.
Microscopy Masters enlists volunteers to do the necessary first step of combing through the photos to find the protein molecules — a time-consuming job that people do better than computers. The task may feel daunting, as each black-and-white image resembles a fuzzy TV screen. Only some of the dark smudges in any given image will be molecules of interest; others will be actual smudges or globs of proteins too jumbled to be of use. Fortunately, a practice tutorial offers a crash course in protein identification. And each image will be classified by many users,
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, alleviating some of the pressure of worrying about marking the wrong thing.
Data from the project will help researchers improve protein-picking computer algorithms, says project member Jacob Bruggemann. That way computers can take over the painstaking work.

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